Mouse anti‑Rat CD163 IgG1 Monoclonal RW-B5967-100

Antibody: CD163 Mouse anti-Rat Monoclonal (ED2) Antibody, Application: IHC, IHC-P, IHC-Fr, IP, Flo, Reactivity: Rat, Format: Unconjugated, Unmodified, Other formats: Biotin FITC HRP PE, Descriptio: CD163 (also called scavenger receptor cysteine-rich type 1 protein M130) is an acute-phase–regulated receptor that is involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages. It has a plasma membrane-bound form and a soluble form. CD163 is expressed on monocytes and mature resident macrophages such as red pulp macrophages in the spleen, Kupffer cells in the liver, alveolar and interstitial macrophages in the lung, medullary and perifollicular macrophages in lymph nodes, and perivascular and meningeal macrophages in the brain. The soluble form may be a biomarker for inflammatory diseases. Antibodies to CD163 can be used to identify monocytes and macrophages. It is positive in histiocytic sarcomas.

References: Diagn Pathol. 2007;2:7. doi: 10.1186/1746-1596-2-7;Biochem Biophys Res Commun. 1999;260(2):466-474. doi: 10.1006/bbrc.1999.0866;J Leukoc Biol. 1999;66(5):858-866. PMID: 10577520;Nature. 2001;409(6817):198-201. doi: 10.1038/35051594;J Leukoc Biol. 2006;79(4):837-845. doi: 10.1189/jlb.1005602;Antioxid Redox Signal. 2013;18(17):2352-2363. doi: 10.1089/ars.2012.4834., Targe: Rat CD163, Synonym: CD163 | CD163 antigen | Macrophage-associated antigen | M130 | CD163 molecule | Hemoglobin scavenger receptor | MM130, Hos: Mouse, Reactivit: Rat (tested or 100% immunogen sequence identity), Clonalit: IgG1 Monoclonal, Clon: ED2, Conjugation: Unconjugated. Also available conjugated with Biotin, FITC, HRP, PE., Purificatio: Protein A purified, Modification: Unmodified, Immunoge: SP2/0-Ag 14 myeloma cells, Specificit: Recognizes rat CD163. Does not react with human., Application: IHC
IHC - Paraffin (10 µg/ml)
IHC - Frozen (1:50 - 1:100)
Immunoprecipitation
Flow Cytometry (1:10 - 1:100), Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: Suitable for use in Flow Cytometry, Immunohistochemistry (frozen and paraffin), and Immunoprecipitation. Flow Cytometry: 1:10-1:100; 10 ul labels 10^6 cells in 100ul. Immunohistochemistry (frozen): 1:50-1:100. Immunohistochemistry (paraffin): Requires antigen retrieval by protein digestion (trypsin or pronase). Reported reaction following PLP fixation (Periodate-lysine-paraformaldehyde)., Presentatio: PBS, pH 7.4, 0.09% Sodium Azide, Storag: Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee., About CD16: Uniprot: Q86VB7 NCBI: NM_004244 NP_004235.4

Cat: RW-73-71609-46