Rabbit anti‑Mouse Collagen II Polyclonal RW-C744051-0.5

Antibody: Collagen II Rabbit anti-Mouse Polyclonal Antibody, Application: IHC-P, IF, WB, ELISA, RIA, Reactivity: Mouse, Cow, Format: Unconjugated, Unmodified, Descriptio: Collagen II antibody RW-C744051 is an unconjugated rabbit polyclonal antibody to Collagen II from mouse. It is reactive with mouse and cow. Validated for ELISA, IF, IHC, RIA and WB., Targe: Mouse Collagen II, Hos: Rabbit, Reactivit: Mouse, Cow (tested or 100% immunogen sequence identity), Clonalit: Polyclonal, Conjugation: Unconjugated, Modification: Unmodified, Immunoge: Purified collagen type II from fetal murine cartilage, Application: IHC - Paraffin (1:1000)
Immunofluorescence (1:80)
Western blot
ELISA (1:200)
Radioimmunoassay, Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 minutes at RT is recommended. Type II collagen is an alpha1(II)-trimer, which forms 67 nm cross-banded fibrils. Typically it can be observed in cartilage and various tumours. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Murine collagen type II 100%, murine collagen type I, III and IV <0.1%, Mouse fibronectin < 0.1%., Presentatio: PBS, Reconstitutio: 0.5 ml Reconstitute in sterile distilled water., Storag: Store at -20°C., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee.

Cat: RW-52-121527-160