Rabbit anti‑Bovine Collagen II Polyclonal RW-C744046-0.1

Antibody: Collagen II Rabbit anti-Bovine Polyclonal Antibody, Application: IHC-P, IF, WB, ELISA, RIA, Reactivity: Bovine, Horse, Pig, Cow, Format: Unconjugated, Unmodified, Descriptio: Collagen II antibody RW-C744046 is an unconjugated rabbit polyclonal antibody to Collagen II from bovine. It is reactive with bovine, cow, horse and other species. Validated for ELISA, IF, IHC, RIA and WB., Targe: Bovine Collagen II, Hos: Rabbit, Reactivit: Bovine, Horse, Pig, Cow (tested or 100% immunogen sequence identity), Clonalit: Polyclonal, Conjugation: Unconjugated, Modification: Unmodified, Immunoge: Purified collagen type II from bovine cartilage, Application: IHC - Paraffin (1:500)
Immunofluorescence (1:40)
Western blot
ELISA (1:200)
Radioimmunoassay, Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 minutes at RT is recommended. Type II collagen is an alpha1(II)-trimer, which forms 67 nm cross-banded fibrils. Typically it can be observed in cartilage and various tumours. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Bovine collagen type II 100%, bovine collagen XI <3.0 %; bovine collagen I and IX <0.1% (RIA at 1:200 dilution)., Presentatio: PBS, Reconstitutio: 0.5 ml Reconstitute in sterile distilled water., Storag: Store at -20°C., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee.

Cat: RW-52-121525-154