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Rabbit anti‑Citrulline Polyclonal RW-C145131-200

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Antibody: Citrulline Rabbit Polyclonal Antibody, Application: ICC, WB, ELISA, Format: Unconjugated, Unmodified, Descriptio: Citrulline antibody RW-C145131 is an unconjugated rabbit polyclonal antibody to Citrulline. Validated for ELISA, ICC and WB., Targe: Citrulline, Hos: Rabbit, Clonalit: Polyclonal, Conjugation: Unconjugated, Purificatio: Antiserum, Modification: Unmodified, Immunoge: Citrulline coupled to KLH via Glutaraldehyde, Specificit: The antibody reacts specifically with intrapeptidic citrulline independently of AA sequence. Does not react with free citrulline, ornithine or arginine., Application: ICC
Western blot
ELISA, Usag: The positive control was HL60 cells treated with DMSO or with DMSO and A23187. The histones of these cells were then extracted.The sample were loaded on a 14% acrylamide SDS PAGE gel.The transfer was made on a PVDF membrane The blocking buffer was PBS 1X with 5% BSA The antibody was used at 1/1000 in a TBT Tween 20 buffer with 1% BSA.The antibody was incubated overnight at +4°C., Presentatio: Lyophilized antiserum, Storag: Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee.

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    Cat: RW-52-121171-123