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Rabbit anti‑Chicken COL9A3 / Collagen IX Polyclonal RW-C744077-0.5
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Antibody: COL9A3 / Collagen IX Rabbit anti-Chicken Polyclonal Antibody, Application: IHC-P, IF, ELISA, RIA, Reactivity: Chicken, Format: Unconjugated, Unmodified, Descriptio: Collagen IX antibody RW-C744077 is an unconjugated rabbit polyclonal antibody to chicken Collagen IX (COL9A3). Validated for ELISA, IF, IHC and RIA., Targe: Chicken COL9A3 / Collagen IX, Synonym: COL9A3 | Collagen alpha 3(x) | Collagen alpha-3(IX) chain | Collagen type IX proteoglycan | EDM3 | DJ885L7.4.1 | IDD | MED | Collagen, type IX, alpha 3, Hos: Rabbit, Reactivit: Chicken (tested or 100% immunogen sequence identity), Clonalit: Polyclonal, Conjugation: Unconjugated, Modification: Unmodified, Immunoge: Purified collagen type IX from chicken breast bone, Application: IHC - Paraffin (1:200 - 1:600)
Immunofluorescence (1:40)
Radioimmunoassay, Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 minutes at RT is recommended. Collagen type IX is found as non-fibrillary structure protein (68-115 kDa) cosisting of alpha-1(IX), alpha-2(IX) and alpha-3(IX) collagen in cartilage and vitreous body. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Chicken collagen type IX 100%, chicken collagen type I, II, III and V negative in ELISA at 1:100 dilution., Presentatio: PBS, Reconstitutio: 0.5 ml Reconstitute in sterile distilled water., Storag: Store at -20°C., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee., About COL9A3 / Collagen I: NCBI: NM_001853

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    Cat: RW-52-103061-186