Rabbit anti‑Human COL3A1 / Collagen III Polyclonal RW-C744081-0.1

Antibody: COL3A1 / Collagen III Rabbit anti-Human Polyclonal Antibody, Application: IHC-P, IF, WB, ELISA, RIA, Reactivity: Human, Pig, Cow, Format: Unconjugated, Unmodified, Descriptio: Collagen III antibody RW-C744081 is an unconjugated rabbit polyclonal antibody to Collagen III (COL3A1) from human. It is reactive with human, cow and pig. Validated for ELISA, IF, IHC, RIA and WB., Targe: Human COL3A1 / Collagen III, Synonym: COL3A1 | Alpha1 (III) collagen | Collagen alpha 1(iii) | Collagen alpha-1(III) chain | Collagen, type III, alpha 1 | Collagen, fetal | EDS4A, Hos: Rabbit, Reactivit: Human, Pig, Cow (tested or 100% immunogen sequence identity), Clonalit: Polyclonal, Conjugation: Unconjugated, Modification: Unmodified, Immunoge: Procollagen type III deprived of the C-Terminus PIIICP peptide, Application: IHC - Paraffin (1:500)
Immunofluorescence (1:40)
Western blot
ELISA (1:100 - 1:200)
Radioimmunoassay (>1:200), Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 minutes at RT is recommended. Collagen type III is synthesized as a homo-trimeric pro-collagen comprising three identical pro-alpha(III)-chains. It has been reported that procollagen type III is processed extracellularly at the ECM and can be found by immunostaining intracellular as well as extracellular. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human and bovine procollagen type III (PIIIP) 100%; human and bovine procollagen type and collagen type I <0.1% in RIA at 1:200 dilution.., Presentatio: PBS, Reconstitutio: 0.1 ml Reconstitute in sterile distilled water., Storag: Store at -20°C., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee., About COL3A1 / Collagen II: Uniprot: P02461 NCBI: NM_000090 NP_000081.1

Cat: RW-52-102502-184