Optimized NTP


Making NTPO: Nuceoside Tri Phophate Optimized Solution for PCR

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Nucleoside 5´-triphosphates (ATP, CTP, GTP, UTP) and a 10 mM NTP Mix that are suitable for use in in vitro transcription reactions with SP6, T3, and T7 RNA polymerases.

 Production of RNA probes or RNA transcripts for translation in in vitro translation extracts.

The key points mentioned in your statement include:


  1. NTPO: Nuceoside Tri Phophate Optimized Solution for PCR
  2. Nucleoside 5´-triphosphates (ATP, CTP, GTP, UTP): These are the building blocks for RNA synthesis. RNA polymerases use these nucleotides to create RNA strands during transcription.
  3. 10 mM NTP Mix: This is likely a mixture of the four nucleoside triphosphates (ATP, CTP, GTP, UTP) at a concentration of 10 mM each. Such mixes provide a convenient and ready-to-use solution for in vitro transcription reactions.
  4. In vitro transcription reactions: SP6, T3, and T7 RNA polymerases are enzymes commonly used for in vitro transcription. They synthesize RNA from a DNA template using nucleoside triphosphates.
  5. Solution composition: The nucleotides are supplied in a solution containing 1 mM Tris-HCl at pH 7.5. Tris-HCl is a buffer commonly used to maintain a stable pH in biochemical reactions.

This information suggests that the provided reagents are suitable for efficiently generating RNA transcripts in an in vitro setting, making them valuable for various applications in molecular biology and biochemistry.


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